DNeasy Blood & Tissue Kit

DNA isolation / purification Tissue - placenta

Experiment
DNA isolation / purification Tissue - placenta
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA Isolation and Bisulfite Treatment
Samples were prepared in GT buffer and stored at −20°C or −80°C. DNA was isolated using the DNeasy Blood&Tissue Kit (Qiagen, Hilden, Germany), according to the manufacturer's protocol. The tissue was homogenized by passing it through a syringe with a 20 G needle a minimum 5–10 times. The DNA was diluted in 100 μl buffer AE (Qiagen). To check quality and to exclude contamination, the DNA product was analyzed by gel electrophoresis. DNA concentrations and protein contamination of the samples were checked using a Nanodrop spectrophotometer (Isogen). Samples with a 260/230 ratio lower than 1.8 were cleaned using standard ethanol precipitation. Depending on the amount of DNA, the samples were resuspended in 20–100 μl buffer AE. After isolation, the samples were stored at 4°C until bisulfite treatment.

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Reviews

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - placenta using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
DNA Methylation and Expression Patterns of Selected Genes in First-Trimester Placental Tissue from Pregnancies with Small-for-Gestational-Age Infants at Birth.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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